Expanding the molecular grammar of polar residues and arginine in FUS prion-like domain phase separation and aggregation.

ABSTRACT: A molecular grammar governing low-complexity prion-like domains phase separation (PS) has been proposed based on mutagenesis experiments that identified tyrosine and arginine as primary drivers of phase separation via aromatic-aromatic and aromatic-arginine interactions. Here we show that additional residues make direct favorable contacts that contribute to phase separation, highlighting the need to account for these contributions in PS theories and models. We find that tyrosine and arginine make important contacts beyond only tyrosine-tyrosine and tyrosine-arginine, including arginine-arginine contacts. Among polar residues, glutamine in particular contributes to phase separation with sequence/position-specificity, making contacts with both tyrosine and arginine as well as other residues,

SUBMITTER: Wake N 

PROVIDER: S-EPMC10888811 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature