Investigating the cis-Regulatory Basis of C₃ and C₄ Photosynthesis in Grasses at Single-Cell Resolution.

ABSTRACT: While considerable knowledge exists about the enzymes pivotal for C₄ photosynthesis, much less is known about the cis-regulation important for specifying their expression in distinct cell types. Here, we use single-cell-indexed ATAC-seq to identify cell-type-specific accessible chromatin regions (ACRs) associated with C₄ enzymes for five different grass species. This study spans four C₄ species, covering three distinct photosynthetic subtypes: Zea mays and Sorghum bicolor (NADP-ME), Panicum miliaceum (NAD-ME), Urochloa fusca (PEPCK), along with the C₃ outgroup Oryza sativa. We studied the cis-regulatory landscape of enzymes essential across all C₄ species and those unique to C₄

SUBMITTER: Mendieta JP 

PROVIDER: S-EPMC10888913 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature