Scalable primordial germ cell-like-cell platform for functional genomics identifies epigenetic fertility modifiers.

ABSTRACT: Primordial germ cells (PGCs) are the founder cells of the germline. The ability to generate PGC-like cells (PGCLCs) from pluripotent stem cells has advanced our knowledge of gametogenesis and holds promise for developing infertility treatments. However, generating an ample supply of PGCLCs for demanding applications such as large-scale genetic screens has been a limitation. Here, we demonstrated that simultaneous overexpression of 4 transcriptional factors - Nanog and three PGC master regulators Prdm1, Prdm14 and Tfap2c - in suspended mouse epiblast like cells (EpiLCs) and formative embryonic stem cells (ESCs) results in efficient and cost-effective production of PGCLCs. Nanog overexpression enhanced the PGC regulatory network, suppressed differentiation

SUBMITTER: Li L 

PROVIDER: S-EPMC10888945 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature