Advanced Surface Passivation for High-Sensitivity Studies of Biomolecular Condensates.

ABSTRACT: Biomolecular condensates are cellular compartments that concentrate biomolecules without an encapsulating membrane. In recent years, significant advances have been made in the understanding of condensates through biochemical reconstitution and microscopic detection of these structures. Quantitative visualization and biochemical assays of biomolecular condensates rely on surface passivation to minimize background and artifacts due to condensate adhesion. However, the challenge of undesired interactions between condensates and glass surfaces, which can alter material properties and impair observational accuracy, remains a critical hurdle. Here, we introduce an efficient, generically applicable, and simple passivation method employing self-assembly of the surfactant Pluronic F127 (PF127). The

SUBMITTER: Yao RW 

PROVIDER: S-EPMC10888978 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature