Integrating Continuous Transepithelial Flux Measurements into an Ussing Chamber Set-Up.

ABSTRACT: Fluorescently labelled compounds are often employed to study the paracellular properties of epithelia. For flux measurements, these compounds are added to the donor compartment and samples collected from the acceptor compartment at regular intervals. However, this method fails to detect rapid changes in permeability. For continuous transepithelial flux measurements in an Ussing chamber setting, a device was developed, consisting of a flow-through chamber with an attached LED, optical filter, and photodiode, all encased in a light-impermeable container. The photodiode output was amplified and recorded. Calibration with defined fluorescein concentration (range of 1 nM to 150 nM) resulted in a linear output. As proof of principle, flux measurements were performed on various cell lines. The re

SUBMITTER: Alija C 

PROVIDER: S-EPMC10889482 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature