Temporal serum neurofilament light chain concentrations in sheep inoculated with the agent of classical scrapie.

ABSTRACT:

Objective

Neurofilament light chain (Nf-L) has been used to detect neuroaxonal damage in the brain caused by physical injury or disease. The purpose of this study was to determine if serum Nf-L could be used as a biomarker for pre-symptomatic detection of scrapie in sheep.

Methods

Four sheep with prion protein genotype AVQQ were intranasally inoculated with the classical scrapie strain x124. Blood was collected every 4 weeks until 44 weeks post-inoculation, at which point weekly collection commenced. Serum was analyzed using single molecule array (Quanterix SR-X) to evaluate Nf-L concentrations.

Results

Scrapie was confirmed in each sheep by testing homogenized brainstem at the level of the obex with a commercially available enzyme immunoassay. Increased serum Nf-L co

SUBMITTER: Brown Q 

PROVIDER: S-EPMC10889644 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature