Approaches to induce the maturation process of human induced pluripotent stem cell derived-endothelial cells to generate a robust model.

ABSTRACT:

Background

Endothelial cells generated from induced pluripotent stem cells (hiPSC-ECs) show the majority of endothelial cell characteristics and markers, such as cobblestone morphology and the expression of VEGF and VE-cadherin. However, these cells are failing to show a mature endothelial cell phenotype, which is represented by the low expression and production of von Willebrand Factor (VWF) leading to the round morphology of the Weibel Palade Bodies (WPBs). The aim of this study was to improve the maturation process of hiPSC-ECs and to increase the levels of VWF.

Methods

hiPSC-ECs were differentiated by a standard differentiation protocol from hiPSCs generated from healthy control donors. To induce maturation, the main focus was to increase the expression and/or production

SUBMITTER: de Boer S 

PROVIDER: S-EPMC10889888 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature