Differential allelic representation (DAR) identifies candidate eQTLs and improves transcriptome analysis.

ABSTRACT: In comparisons between mutant and wild-type genotypes, transcriptome analysis can reveal the direct impacts of a mutation, together with the homeostatic responses of the biological system. Recent studies have highlighted that, when the effects of homozygosity for recessive mutations are studied in non-isogenic backgrounds, genes located proximal to the mutation on the same chromosome often appear over-represented among those genes identified as differentially expressed (DE). One hypothesis suggests that DE genes chromosomally linked to a mutation may not reflect functional responses to the mutation but, instead, result from an unequal distribution of expression quantitative trait loci (eQTLs) between sample groups of mutant or wild-type genotypes. This is problematic because eQTL expressio

SUBMITTER: Baer L 

PROVIDER: S-EPMC10890730 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature