Building Fluorescence Lifetime Maps Photon-by-Photon by Leveraging Spatial Correlations.

ABSTRACT: Fluorescence lifetime imaging microscopy (FLIM) has become a standard tool in the quantitative characterization of subcellular environments. However, quantitative FLIM analyses face several challenges. First, spatial correlations between pixels are often ignored as signal from individual pixels is analyzed independently thereby limiting spatial resolution. Second, existing methods deduce photon ratios instead of absolute lifetime maps. Next, the number of fluorophore species contributing to the signal is unknown, while excited state lifetimes with <1 ns difference are difficult to discriminate. Finally, existing analyses require high photon budgets and often cannot rigorously propagate experimental uncertainty into values over lifetime maps and number of species involved. To overcome all o

SUBMITTER: Fazel M 

PROVIDER: S-EPMC10890823 | biostudies-literature | 2023 Oct

REPOSITORIES: biostudies-literature