Runx1+ vascular smooth muscle cells are essential for hematopoietic stem and progenitor cell development in vivo.

ABSTRACT: Hematopoietic stem cells (HSCs) produce all essential cellular components of the blood. Stromal cell lines supporting HSCs follow a vascular smooth muscle cell (vSMC) differentiation pathway, suggesting that some hematopoiesis-supporting cells originate from vSMC precursors. These pericyte-like precursors were recently identified in the aorta-gonad-mesonephros (AGM) region; however, their role in the hematopoietic development in vivo remains unknown. Here, we identify a subpopulation of NG2⁺Runx1⁺ perivascular cells that display a sclerotome-derived vSMC transcriptomic profile. We show that deleting Runx1 in NG2⁺ cells impairs the hematopoietic development in vivo and causes transcriptional changes in pericytes/vSMCs, endothelial cells and hematopoietic cel

SUBMITTER: Gonzalez Galofre ZN 

PROVIDER: S-EPMC10891074 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature