Single-cell transcriptomics identifies the differentiation trajectory from inflammatory monocytes to pro-resolving macrophages in a mouse skin allergy model.

ABSTRACT: Both monocytes and macrophages are heterogeneous populations. It was traditionally understood that Ly6C^hi classical (inflammatory) monocytes differentiate into pro-inflammatory Ly6C^hi macrophages. Accumulating evidence has suggested that Ly6C^hi classical monocytes can also differentiate into Ly6C^lo pro-resolving macrophages under certain conditions, while their differentiation trajectory remains to be fully elucidated. The present study with scRNA-seq and flow cytometric analyses reveals that Ly6C^hiPD-L2^lo classical monocytes recruited to the allergic skin lesion sequentially differentiate into Ly6C^loPD-L2^hi pro-resolving macrophages, via intermediate Ly6C^hiPD-L2^hi macrophages but

SUBMITTER: Miyake K 

PROVIDER: S-EPMC10891131 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature