Bulk RNA sequencing reveals the comprehensive genetic characteristics of human cord blood-derived natural killer cells.

ABSTRACT:

Introduction

Innate immune cells are important in tumor immunotherapy. Natural killer cells (NKCs) are also categorized as innate immune cells and can control tumor growth and metastatic spread. Glioblastoma (GBM) is the most common and aggressive primary brain tumor in adults. NKC-based immunotherapy is a promising treatment strategy against GBM. We previously reported a feeder-free expansion system that yielded large-scale highly purified and cytotoxic NKCs derived from human cord blood (CB). In the present study, we performed comprehensive genomic analyses of NKCs generated from human CB (CBNKCs) as compared those from human peripheral blood (PB) (PBNKCs).

Methods

Frozen T cell-free CB mononuclear cells were cultured with recombinant human interleukin (rhIL)-18 and rhIL-2

SUBMITTER: Morimoto T 

PROVIDER: S-EPMC10891285 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature