A Pillar and Perfusion Plate Platform for Robust Human Organoid Culture and Analysis.

ABSTRACT: Human organoids have the potential to revolutionize in vitro disease modeling by providing multicellular architecture and function that are similar to those in vivo. This innovative and evolving technology, however, still suffers from assay throughput and reproducibility to enable high-throughput screening (HTS) of compounds due to cumbersome organoid differentiation processes and difficulty in scale-up and quality control. Using organoids for HTS is further challenged by the lack of easy-to-use fluidic systems that are compatible with relatively large organoids. Here, these challenges are overcome by engineering "microarray three-dimensional (3D) bioprinting" technology and associated pillar and perfusion plates for human organoid culture and analysis. High-precision, high-throughput stem

SUBMITTER: Kang SY 

PROVIDER: S-EPMC10891301 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature