Endogenous monoclonal immunoglobulins analyzed using the EXENT® solution and LC-MS.

ABSTRACT:

Introduction

The EXENT® Solution, a fully automated system, is a recent advancement for identifying and quantifying monoclonal immunoglobulins in serum. It combines immunoprecipitation with MALDI-TOF mass spectrometry. Compared to gel-based methods, like SPEP and IFE, it has demonstrated the ability to detect monoclonal immunoglobulins in serum at lower levels. In this study, samples that tested negative using EXENT® were reflexed to LC-MS to determine if the more sensitive LC-MS method could identify monoclonal immunoglobulins missed by EXENT®.

Objectives

To assess whether monoclonal immunoglobulins that are not detected by EXENT® can be detected by LC-MS using a low flow LC system coupled to a Q-TOF mass spectrometer.

Methods

Samples obtained from patients confirmed

SUBMITTER: Barnidge D 

PROVIDER: S-EPMC10891330 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature