In-vitro and preclinical testing of <i>bacillus subtilis</i> UBBS-14 probiotic in rats shows no toxicity.

ABSTRACT:

Introduction

Probiotics made from Bacillus subtilis provide a wide spread of health benefits, particularly in the treatment of diarrhea and gastrointestinal problems. Herein, we employed in vitro and in vivo paradigms to assess the potential adverse effects and toxicity of B. subtilis UBBS-14.

Materials and methods

According to Organization for Economic Co-operation and Development (OECD) 423 and 407 requirements, a preclinical investigation was conducted in male and female Sprague-Dawley rats. Acute toxicity was examined following a single peroral (PO) administration of 5,000 mg/kg body weight (bw) i.e. equivalent to 500 billion colony-forming units (CFU) per kg bw. Single administration of B. subtilis UBBS-14 showed no mortality or adverse effects unti

SUBMITTER: Negi A 

PROVIDER: S-EPMC10891425 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature