Bacterial Adaptive Memory in Methicillin-Resistant <i>Staphylococcus aureus</i> from Endotracheal Tubes.

ABSTRACT:

Objectives

To evaluate the expression dynamics of biofilm genes in methicillin-resistant Staphylococcus aureus (MRSA) retrieved from endotracheal tubes (ETT) and to determine how gene regulation is attenuated in vitro where host-environmental factors are no longer present.

Methods

Biofilm was grown (24 h) in tryptic broth soy plus 0.25% glucose for a clinical MRSA isolate in planktonic state and after sessile growth named ETT-MRSA (S2, S3, S4, S5, S6, S7). Gene expression of five biofilm-related genes (icaC, clfB, ebps, fnbB, and RNA III) was assessed consecutively from day 1 to day 4 after ETT growth through real-time PCR. 16S rRNA was used as a control.

Results

The MRSA isolates retrieved from ETT were capable of producing b

SUBMITTER: Fernandez-Barat L 

PROVIDER: S-EPMC10892081 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature