HIV-1 Proviral Genome Engineering with CRISPR-Cas9 for Mechanistic Studies.

ABSTRACT: HIV-1 latency remains a barrier to a functional cure because of the ability of virtually silent yet inducible proviruses within reservoir cells to transcriptionally reactivate upon cell stimulation. HIV-1 reactivation occurs through the sequential action of host transcription factors (TFs) during the "host phase" and the viral TF Tat during the "viral phase", which together facilitate the positive feedback loop required for exponential transcription, replication, and pathogenesis. The sequential action of these TFs poses a challenge to precisely delineate the contributions of the host and viral phases of the transcriptional program to guide future mechanistic and therapeutic studies. To address this limitation, we devised a genome engineering approach to mutate tat and create a gene

SUBMITTER: Hyder U 

PROVIDER: S-EPMC10892460 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature