Dataset Information

Cloning, Expression, and Functional Analysis of the MYB Transcription Factor SlMYB86-like in Tomato.

ABSTRACT: MYB transcription factors (TFs) have been shown to play a key role in plant growth and development and are in response to various types of biotic and abiotic stress. Here, we clarified the structure, expression patterns, and function of a MYB TF, SlMYB86-like (Solyc06g071690) in tomato using an inbred tomato line exhibiting high resistance to bacterial wilt (Hm 2-2 (R)) and one susceptible line (BY 1-2 (S)). The full-length cDNA sequence of this gene was 1226 bp, and the open reading frame was 966 bp, which encoded 321 amino acids; its relative molecular weight was 37.05055 kDa; its theoretical isoelectric point was 7.22; it was a hydrophilic nonsecreted protein; and it had no transmembrane structures. The protein also contains a highly conserved MYB DNA-binding domain and was predicted to be localized to the nucleus. Phylogenetic analysis revealed that SlMYB86-like is closely related to SpMYB86-like in Solanum pennellii and clustered with other members of the family Solanaceae. Quantitative real-time PCR (qRT-PCR) analysis revealed that the expression of the SlMYB86-like gene was tissue specific and could be induced by Ralstonia solanacearum, salicylic acid, and jasmonic acid. The results of virus-induced gene silencing (VIGS) revealed that SlMYB86-like silencing decreased the resistance of tomato plants to bacterial wilt, suggesting that it positively regulates the resistance of tomatoes to bacterial wilt. Overall, these findings indicate that SlMYB86-like plays a key role in regulating the resistance of tomatoes to bacterial wilt.

SUBMITTER: Chen N

PROVIDER: S-EPMC10893056 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature

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Cloning, Expression, and Functional Analysis of the MYB Transcription Factor SlMYB86-like in Tomato.

Chen Na N Zhan Wenwen W Shao Qin Q Liu Liangliang L Lu Qineng Q Yang Weihai W Que Zhiqun Z

Plants (Basel, Switzerland) 20240208 4

MYB transcription factors (TFs) have been shown to play a key role in plant growth and development and are in response to various types of biotic and abiotic stress. Here, we clarified the structure, expression patterns, and function of a MYB TF, SlMYB86-like (Solyc06g071690) in tomato using an inbred tomato line exhibiting high resistance to bacterial wilt (Hm 2-2 (R)) and one susceptible line (BY 1-2 (S)). The full-length cDNA sequence of this gene was 1226 bp, and the open readi ...[more]

PMID: 38498460

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Dataset Information

Cloning, Expression, and Functional Analysis of the MYB Transcription Factor SlMYB86-like in Tomato.

Publications

Cloning, Expression, and Functional Analysis of the MYB Transcription Factor <i>SlMYB86-like</i> in Tomato.

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