Genetic and Biochemical Characterization of AXC-2 from <i>Achromobacter ruhlandii</i>.

ABSTRACT: Achromobacter spp. are intrinsically resistant to multiple antibiotics and can also acquire resistance to those commonly used for the treatment of respiratory infections, especially in patients with cystic fibrosis. The aim of this study was to perform the genetic and biochemical characterization of AXC-2 from A. ruhlandii and to analyze all available AXC variants. Steady-state kinetic parameters were determined on a purified AXC-2 enzyme. It exhibited higher catalytic efficiencies towards amino-penicillins and older cephalosporins, while carbapenems behaved as poor substrates. Phylogenetic analysis of all bla_AXC variants available in the NCBI was conducted. AXC was encoded in almost all A. ruhlandii genomes, whereas it was only found in 30% of A.

SUBMITTER: Papalia M 

PROVIDER: S-EPMC10893412 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature