Unlocking Nature's Toolbox: glutamate-inducible recombinant protein production from the Komagatella phaffii PEPCK promoter.

ABSTRACT:

Background

Komagataella phaffii (a.k.a. Pichia pastoris) harbors a glutamate utilization pathway in which synthesis of glutamate dehydrogenase 2 and phosphoenolpyruvate carboxykinase (PEPCK) is induced by glutamate. Glutamate-inducible synthesis of these enzymes is regulated by Rtg1p, a cytosolic, basic helix-loop-helix protein. Here, we report food-grade monosodium glutamate (MSG)-inducible recombinant protein production from K. phaffii PEPCK promoter (P_PEPCK) using green fluorescent protein (GFP) and receptor binding domain of SARS-CoV-2 virus (RBD) as model proteins.

Results

P_PEPCK-RBD/GFP expression cassette was integrated at two different sites in the genome to improve recombinant protein yield from P_PEPCK. The traditional, methanol-indu

SUBMITTER: Rajak N 

PROVIDER: S-EPMC10893637 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature