Deficiency of Acetyltransferase nat10 in Zebrafish Causes Developmental Defects in the Visual Function.

ABSTRACT:

Purpose

N4-acetylcytidine (ac4C) is a post-transcriptional RNA modification catalyzed by N-acetyltransferase 10 (NAT10), a critical factor known to influence mRNA stability. However, the role of ac4C in visual development remains unexplored.

Methods

Analysis of public datasets and immunohistochemical staining were conducted to assess the expression pattern of nat10 in zebrafish. We used CRISPR/Cas9 and RNAi technologies to knockout (KO) and knockdown (KD) nat10, the zebrafish ortholog of human NAT10, and evaluated its effects on early development. To assess the impact of nat10 knockdown on visual function, we performed comprehensive histological evaluations and behavioral analyses. Transcriptome profiling and real-time (RT)-PCR were utilized to detect alterations in gene exp

SUBMITTER: Yang HZ 

PROVIDER: S-EPMC10893899 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature