Impact of Nintedanib and Anti-Angiogenic Agents on Uveal Melanoma Cell Behavior.

ABSTRACT:

Purpose

The purpose of this study was to investigate the direct impact of the combined angiokinase inhibitor nintedanib as well as the anti-angiogenic agents ranibizumab, bevacizumab, and aflibercept on the primary uveal melanoma (UM) cell line Mel270 and liver metastasis UM cell line OMM2.5.

Methods

The metabolic activity, viability, and oxidative stress levels were analyzed by the Thiazolyl Blue Tetrazolium Bromide (MTT), LIVE/DEAD, and reactive oxygen species (ROS) assays. Expression of intracellular VEGF-A165 and VEGF receptor-2 was detected by immunofluorescent staining. The secretion of VEGF-A165 into the cell culture supernatants was evaluated by VEGF-A165 ELISA.

Results

Nintedanib, at a concentration of 1 µg/mL, resulted in a median reduction of metabolic acti

SUBMITTER: Pawlik VE 

PROVIDER: S-EPMC10893901 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature