Fine-tuning rice heading date through multiplex editing of the regulatory regions of key genes by CRISPR-Cas9.

ABSTRACT: Heading date (or flowering time) is a key agronomic trait that affects seasonal and regional adaption of rice cultivars. An unoptimized heading date can either not achieve a high yield or has a high risk of encountering abiotic stresses. There is a strong demand on the mild to moderate adjusting the heading date in breeding practice. Genome editing is a promising method which allows more precise and faster changing the heading date of rice. However, direct knock out of major genes involved in regulating heading date will not always achieve a new germplasm with expected heading date. It is still challenging to quantitatively adjust the heading date of elite cultivars with best adaption for broader region. In this study, we used a CRISPR-Cas9 based genome editing strategy called high-efficie

SUBMITTER: Zhou S 

PROVIDER: S-EPMC10893950 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature