A streamlined approach to structure elucidation using in cellulo crystallized recombinant proteins, InCellCryst.

ABSTRACT: With the advent of serial X-ray crystallography on microfocus beamlines at free-electron laser and synchrotron facilities, the demand for protein microcrystals has significantly risen in recent years. However, by in vitro crystallization extensive efforts are usually required to purify proteins and produce sufficiently homogeneous microcrystals. Here, we present InCellCryst, an advanced pipeline for producing homogeneous microcrystals directly within living insect cells. Our baculovirus-based cloning system enables the production of crystals from completely native proteins as well as the screening of different cellular compartments to maximize chances for protein crystallization. By optimizing cloning procedures, recombinant virus production, crystallization and crystal detection, X-ray di

SUBMITTER: Schonherr R 

PROVIDER: S-EPMC10894269 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature