"High-throughput screening of catalytically active inclusion bodies using laboratory automation and Bayesian optimization".

ABSTRACT:

Background

In recent years, the production of inclusion bodies that retain substantial catalytic activity was demonstrated. These catalytically active inclusion bodies (CatIBs) are formed by genetic fusion of an aggregation-inducing tag to a gene of interest via short linker polypeptides. The resulting CatIBs are known for their easy and cost-efficient production, recyclability as well as their improved stability. Recent studies have outlined the cooperative effects of linker and aggregation-inducing tag on CatIB activities. However, no a priori prediction is possible so far to indicate the best combination thereof. Consequently, extensive screening is required to find the best performing CatIB variant.

Results

In this work, a semi-automated cloning workflow was implemented

SUBMITTER: Helleckes LM 

PROVIDER: S-EPMC10894497 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature