Detection of PSMA expression on circulating tumor cells by blood-based liquid biopsy in prostate cancer.

ABSTRACT:

Background

For patients with mCRPC, PSMA-targeted radioligand treatment has significantly improved the clinical outcome. A blood-based liquid biopsy assay for recognizing PSMA protein expression on circulating tumor cells may be beneficial for better informing therapeutic decision-making and identifying the patients most likely to benefit from PSMA-targeted radioligand therapy.

Methods

Using high-throughput imaging and digital AI pathology algorithms, a four-color immunofluorescence assay has been developed to find PSMA protein expression on CTCs on a glass slide. Cell line cells (LNCaP/PC3s/22Rv1) spiked into healthy donor blood were used to study the precision, specificity, sensitivity, limit of detection, and overall accuracy of the assay. Clinical validation and low-pass

SUBMITTER: Gupta S 

PROVIDER: S-EPMC10895373 | biostudies-literature | 2024 Jan-Dec

REPOSITORIES: biostudies-literature