Mass spectrometry-based study defines the human urine nitrolipidome.

ABSTRACT: Nitrated fatty acids (NO₂-FA) are an endogenous class of signaling mediators formed mainly during digestion and inflammation. The signaling actions of NO₂-FA have been extensively studied, but their detection and characterization lagged. Several different nitrated fatty acid species have been reported in animals and humans, but their formation remains controversial, and a systemic approach to define the endogenous pool of NO₂-FA is needed. Herein, we screened for endogenous NO₂-FA in urine from healthy human volunteers as this is the main excretion route for NO₂-FA and its metabolites, and it provides an excellent matrix for evaluation. Only isomers of two fatty acids, conjugated linoleic and linolenic acid were found to be nitrated. S

SUBMITTER: Salvatore SR 

PROVIDER: S-EPMC10895545 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature