A Fluorescent Sex-Sorting Technique for Insects with the Demonstration in Drosophila melanogaster.

ABSTRACT: Recent advances in insect genetic engineering offer alternative genetic biocontrol solutions to control populations of pests and disease vectors. While success has been achieved, sex-sorting remains problematic for scaling many genetic biocontrol interventions. Here, we describe the development of a genetically stable sex-sorting technique for female and male selection with a proof of concept in Drosophila melanogaster termed SEPARATOR (Sexing Element Produced by Alternative RNA-splicing of A Transgenic Observable Reporter). This elegant approach utilizes dominantly expressed fluorescent proteins and differentially spliced introns to ensure sex-specific expression. The system has the potential for adaptability to various insect species and application for high-throughput insect sex-

SUBMITTER: Liu J 

PROVIDER: S-EPMC10895710 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature