Single-cell RNA sequencing reveals roles of unique retinal microglia types in early diabetic retinopathy.

ABSTRACT:

Background

The pathophysiological mechanisms of diabetic retinopathy (DR), a blinding disease, are intricate. DR was thought to be a microvascular disease previously. However, growing studies have indicated that the retinal microglia-induced inflammation precedes microangiopathy. The binary concept of microglial M1/M2 polarization paradigms during inflammatory activation has been debated. In this study, we confirmed microglia had the most significant changes in early DR using single-cell RNA sequencing.

Methods

A total of five retinal specimens were collected from donor SD rats. Changes in various cells of the retina at the early stage of DR were analyzed using single-cell sequencing technology.

Results

We defined three new microglial subtypes at cellular level, inclu

SUBMITTER: Wang Y 

PROVIDER: S-EPMC10895757 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature