The effects of low-dose IL-2 on Th17/Treg cell imbalance in primary biliary cholangitis mouse models.

ABSTRACT:

Background/aims

Primary biliary cholangitis (PBC) is a chronic cholestatic liver disease. The imbalance of Th17/Treg cells has been reported in PBC patients. Low-dose IL-2 can alleviate disease severity through modulating CD4 + T cell subsets in patients with autoimmune diseases. Hence, the present study aimed to examine the effects and mechanism of low-dose IL-2 in PBC mouse models.

Methods

PBC models were induced in female C57BL/6 mice by two immunizations with 2OA-BSA at two-week intervals, and poly I: C every three days. PBC mouse models were divided into the IL-2 treated and untreated groups and low-dose IL-2 was injected at three different time points. Th17 and Tregs were analyzed by flow cytometry, and the related cytokines were analyzed by ELISA. Liver histopathology

SUBMITTER: Wang Z 

PROVIDER: S-EPMC10895794 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature