3D printed microfluidic devices for integrated solid-phase extraction and microchip electrophoresis of preterm birth biomarkers.

ABSTRACT:

Background

Preterm birth (PTB) is a leading cause of neonatal mortality, such that the need for a rapid and accurate assessment for PTB risk is critical. Here, we developed a 3D printed microfluidic system that integrated solid-phase extraction (SPE) and microchip electrophoresis (μCE) of PTB biomarkers, enabling the combination of biomarker enrichment and labeling with μCE separation and fluorescence detection.

Results

Reversed-phase SPE monoliths were photopolymerized in 3D printed devices. Microvalves in the device directed sample between the SPE monolith and the injection cross-channel in the serpentine μCE channel. Successful on-chip preconcentration, labeling and μCE separation of four PTB-related polypeptides were demonstrated in these integrated microfluidic devices.

SUBMITTER: Esene JE 

PROVIDER: S-EPMC10895869 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature