RGS6 drives cardiomyocyte death following nucleolar stress by suppressing Nucleolin/miRNA-21.

ABSTRACT:

Background

Prior evidence demonstrated that Regulator of G protein Signaling 6 (RGS6) translocates to the nucleolus in response to cytotoxic stress though the functional significance of this phenomenon remains unknown.

Methods

Utilizing in vivo gene manipulations in mice, primary murine cardiac cells, human cell lines and human patient samples we dissect the participation of a RGS6-nucleolin complex in chemotherapy-dependent cardiotoxicity.

Results

Here we demonstrate that RGS6 binds to a key nucleolar protein, Nucleolin, and controls its expression and activity in cardiomyocytes. In the human myocyte AC-16 cell line, induced pluripotent stem cell derived cardiomyocytes, primary murine cardiomyocytes, and the intact murine myocardium tuning RGS6 levels via overexpress

SUBMITTER: Sengar AS 

PROVIDER: S-EPMC10895901 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature