Uncovering Structural Plasticity of <i>Enterovirus A</i> through Deep Insertional and Deletional Scanning.

ABSTRACT: Insertions and deletions (InDels) are essential sources of novelty in protein evolution. In RNA viruses, InDels cause dramatic phenotypic changes contributing to the emergence of viruses with altered immune profiles and host engagement. This work aimed to expand our current understanding of viral evolution and explore the mutational tolerance of RNA viruses to InDels, focusing on Enterovirus A71 (EV-A71) as a prototype for Enterovirus A species (EV-A). Using newly described deep InDel scanning approaches, we engineered approximately 45,000 insertions and 6,000 deletions at every site across the viral proteome, quantifying their effects on viral fitness. As a general trend, most InDels were lethal to the virus. However, our screen reproducibly identified a set of InDel-tolerant regions, dem

SUBMITTER: Bakhache W 

PROVIDER: S-EPMC10896406 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature