Quasi-spherical silver nanoparticles for human prolactin detection by surface-enhanced Raman spectroscopy.

ABSTRACT: Prolactin is a polypeptide hormone made of 199 amino acids; 50% of the amino acid chain forms helices, and the rest forms loops. This hormone is typically related to initiating and maintaining lactation, although it is also elevated in various pathological conditions. Serum prolactin levels of 2 to 18 ng ml^-1 in men, up to 30 ng ml^-1 in women, and 10 to 210 ng ml^-1 in pregnant women are considered normal. Immunoassay techniques used for detection are susceptible to error in different clinical conditions. Surface-enhanced Raman spectroscopy (SERS) is a technique that allows for obtaining the protein spectrum in a simple, fast, and reproducible manner. Nonetheless, proper characterization of human prolactin's Raman/SERS spectrum at different concentrations ha

SUBMITTER: Ortiz-Dosal A 

PROVIDER: S-EPMC10897535 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature