Dataset Information

Isolated Lactobacillus fermentum Ab.RS22 from traditional dairy products inhibits HeLa cervical cancer cell proliferation and modulates apoptosis by the PTEN-Akt pathway.

ABSTRACT:

Objectives

It is worthwhile to note that, some probiotics such as Lactobacilli and Bifidobacteria isolated from dairy products have significant therapeutic effects against cancer cells. Here, we evaluated anti-proliferation and the apoptotic effects of isolated Lactobacillus fermentum Ab.RS22 from traditional dairy products on the HeLa cervical cancer cells in vitro.

Materials and methods

The viability of treated HeLa cells with supernatant of Lactobacillus in 0.5, 0.75, 1, 1.5, and 2 ng/ml concentrations, and IC₅₀ values were detected by tetrazolium bromide. The L. fermentum Ab.RS22-induced cell death by ﬂow cytometry was confirmed through evaluation of the expression of caspase-3, P53, PTEN, and AKT genes by quantitative reverse transcription-polymerase chain reactions (qRT-PCR).

Results

Most cytotoxicity effects of Lactobacillus on HeLa cells were detected in 2 ng/ml at 24 hr (P<0.01); also, the IC₅₀ value was measured as 1.5 ng/ml. The findings of the flow cytometry assay showed that L. fermentum Ab.RS22 in 1.5 ng/ml concentration at 24 hr increased the percentage of both apoptosis and necrosis cells. Lactobacillus-induced cell death was verified through results of Real-time PCR; where expression of caspase-3, P53, and PTEN genes was increased (P<0.01), and also expression of AKT gene (anti-apoptotic) was decreased (P<0.05).

Conclusion

Our findings showed that L. fermentum Ab.RS22 could dose-dependently inhibit the proliferation of the HeLa cells. Its apoptotic effect was confirmed via modulating PTEN/p53/Akt gene expression and activation of the caspase-3 mediated apoptosis pathway. Therefore, L. fermentum Ab.RS22 can be considered a valuable anticancer candidate against cervical cancer progression in subsequent studies.

SUBMITTER: Asoudeh-Fard A

PROVIDER: S-EPMC10897561 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature

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Publications

Isolated <i>Lactobacillus fermentum</i> Ab.RS22 from traditional dairy products inhibits HeLa cervical cancer cell proliferation and modulates apoptosis by the PTEN-Akt pathway.

Asoudeh-Fard Abbas A Salehi Mitra M Ilghari Dariush D Parsaei Asghar A Heydarian Peyman P Piri Hossein H

Iranian journal of basic medical sciences 20240101 4

<h4>Objectives</h4>It is worthwhile to note that, some probiotics such as <i>Lactobacilli</i> and <i>Bifidobacteria</i> isolated from dairy products have significant therapeutic effects against cancer cells. Here, we evaluated anti-proliferation and the apoptotic effects of isolated <i>Lactobacillus fermentum</i> Ab.RS22 from traditional dairy products on the HeLa cervical cancer cells <i>in vitro.</i><h4>Materials and methods</h4>The viability of treated HeLa cells with supernatant of <i>Lactob ...[more]

PMID: 38419886

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