Rational Approach to Optimizing Conformation-Switching Aptamers for Biosensing Applications.

ABSTRACT: The utilization of structure-switching aptamers (SSAs) has enabled the development of novel sensing platforms for the sensitive and continuous detection of molecules. De novo development of SSAs, however, is complex and laborious. Here we describe a rational approach to SSA optimization that simultaneously improves aptamer binding affinity and introduces target-dependent conformation-switching for compatibility with real-world biosensor applications. Key structural features identified from NMR and computational modeling were used to optimize conformational switching in the presence of target, while large-scale, microarray-based mutation analysis was used to map regions of the aptamer permissive to mutation and identify combinations of mutations with stronger binding affinity. Optimi

SUBMITTER: Wolfe M 

PROVIDER: S-EPMC10897929 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature