Cluster-Enhanced Nanopore Sensing of Ovarian Cancer Marker Peptides in Urine.

ABSTRACT: The development of novel methodologies that can detect biomarkers from cancer or other diseases is both a challenge and a need for clinical applications. This partly motivates efforts related to nanopore-based peptide sensing. Recent work has focused on the use of gold nanoparticles for selective detection of cysteine-containing peptides. Specifically, tiopronin-capped gold nanoparticles, trapped in the cis-side of a wild-type α-hemolysin nanopore, provide a suitable anchor for the attachment of cysteine-containing peptides. It was recently shown that the attachment of these peptides onto a nanoparticle yields unique current signatures that can be used to identify the peptide. In this article, we apply this technique to the detection of ovarian cancer marker peptides ranging in length from

SUBMITTER: Rockett TW 

PROVIDER: S-EPMC10897939 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature