Identification and functional characterisation of a locus for target site integration in Fusarium graminearum.

ABSTRACT:

Background

Fusarium Head Blight (FHB) is a destructive floral disease of different cereal crops. The Ascomycete fungus Fusarium graminearum (Fg) is one of the main causal agents of FHB in wheat and barley. The role(s) in virulence of Fg genes include genetic studies that involve the transformation of the fungus with different expression cassettes. We have observed in several studies where Fg genes functions were characterised that integration of expression cassettes occurred randomly. Random insertion of a cassette may disrupt gene expression and/or protein functions and hence the overall conclusion of the study. Target site integration (TSI) is an approach that consists of identifying a chromosomal region where the cassette can be inserted. The identification of a suitable locus f

SUBMITTER: Darino M 

PROVIDER: S-EPMC10898126 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature