Phase contrast reflectance confocal brain imaging at 1650 nm.

ABSTRACT:

Significance

The imaging depth of microscopy techniques is limited by the ability of light to penetrate biological tissue. Recent research has addressed this limitation by combining a reflectance confocal microscope with the NIR-II (or shortwave infrared) spectrum. This approach offers significant imaging depth, is straightforward in design, and remains cost-effective. However, the imaging system, which relies on intrinsic signals, could benefit from adjustments in its optical design and post-processing methods to differentiate cortical cells, such as neurons and small blood vessels.

Aim

We implemented a phase contrast detection scheme to a reflectance confocal microscope using NIR-II spectral range as illumination.

Approach

We analyzed the features retrieved in the i

SUBMITTER: Martel PD 

PROVIDER: S-EPMC10898133 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature