Therapeutic drug monitoring of clozapine in human serum by high-throughput paper spray mass spectrometry.

ABSTRACT:

Introduction

Monitoring the atypical antipsychotic drug clozapine is crucial to ensure patient safety. This article showcases a high-throughput analytical method for measuring clozapine and its primary metabolite norclozapine (N-desmethylclozapine) in serum using paper spray mass spectrometry (PS-MS).

Objectives

This study aimed to assess the viability of a PS-MS method for the rapid measurement of clozapine and norclozapine in human serum samples as an alternative to liquid chromatography mass spectrometry (LC-MS).

Methods

Serum samples were processed by protein precipitation followed by deposition of the supernatant containing labelled internal standards onto paper spray substrates mounted in cartridges. Analytes were then analyzed using a triple quadrupole mass spe

SUBMITTER: Saatchi A 

PROVIDER: S-EPMC10899010 | biostudies-literature | 2024 Apr

REPOSITORIES: biostudies-literature