Polyphenol exposure of mothers and infants assessed by LC-MS/MS based biomonitoring in breast milk.

ABSTRACT: Exposure to polyphenols is relevant throughout critical windows of infant development, including the breastfeeding phase. However, the quantitative assessment of polyphenols in human breast milk has received limited attention so far, though polyphenols may positively influence infant health. Therefore, a targeted LC-MS/MS assay was developed to investigate 86 analytes representing different polyphenol classes in human breast milk. The sample preparation consisted of liquid extraction, salting out, freeze-out, and a dilution step. Overall, nearly 70% of the chemically diverse polyphenols fulfilled all strict validation criteria for full quantitative assessment. The remaining analytes did not fulfill all criteria at every concentration level, but can still provide useful semi-quantitative in

SUBMITTER: Berger S 

PROVIDER: S-EPMC10899372 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature