Endothelial-derived small extracellular vesicles support B-cell acute lymphoblastic leukemia development.

ABSTRACT:

Purpose

The bone marrow niche plays an important role in leukemia development. However, the contributions of different niche components to leukemia development and their underlying mechanisms remain largely unclear.

Method

Cre/LoxP-based conditional knockout technology was used to delete VPS33B or ANGPTL2 gene in niche cells. Murine B-ALL model was established by overexpressing the N-Myc oncogene in hematopoietic stem progenitor cells. The frequency of leukemia cells and immunophenotypic B220⁺ CD43⁺ LICs was detected by flow cytometry. SEVs was isolated by sequential centrifugation and mass spectrometry was performed to analyze the different components of SEVs. Immunoprecipitation and western blot were used to measure the interaction of VPS33B and ANGPT

SUBMITTER: Huang D 

PROVIDER: S-EPMC10899377 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature