MiR-939-3p induces sarcoma proliferation and poor prognosis via suppressing BATF2.

ABSTRACT:

Background

Sarcoma is a rare and aggressive malignancy with poor prognosis, in which oncogene activation and tumor suppressor inactivation are involved. Accumulated studies suggested basic leucine zipper transcription factor ATF-like 2 (BATF2) as a candidate tumor suppressor, but its specific role and mechanism in sarcoma remain unclear.

Methods

The expression levels of BATF2 and miR-939-3p were evaluated by using human sarcoma samples, cell lines and xenograft mouse models. Bioinformatics analysis, qPCR, Western blot, cell proliferation assay, overexpression plasmid construction, point mutation and dual luciferase reporter assay were utilized to investigate the role and mechanism of miR-939-3p in sarcoma.

Results

In this study, we demonstrated that the expression of

SUBMITTER: Xu W 

PROVIDER: S-EPMC10899471 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature