Quantitative, titratable and high-throughput reporter assays to measure DNA double strand break repair activity in cells.

ABSTRACT: Repair of DNA damage is essential for the maintenance of genome stability and cell viability. DNA double strand breaks (DSBs) constitute a toxic class of DNA lesion and multiple cellular pathways exist to mediate their repair. Robust and titratable assays of cellular DSB repair (DSBR) are important to functionally interrogate the integrity and efficiency of these mechanisms in disease models as well as in response to genetic or pharmacological perturbations. Several variants of DSBR reporters are available, however these are often limited by throughput or restricted to specific cellular models. Here, we describe the generation and validation of a suite of extrachromosomal reporter assays that can efficiently measure the major DSBR pathways of homologous recombination (HR), classical nonhom

SUBMITTER: Rajendra E 

PROVIDER: S-EPMC10899754 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature