Enhancer-promoter interactions can form independently of genomic distance and be functional across TAD boundaries.

ABSTRACT: Topologically Associating Domains (TADs) have been suggested to facilitate and constrain enhancer-promoter interactions. However, the role of TAD boundaries in effectively restricting these interactions remains unclear. Here, we show that a significant proportion of enhancer-promoter interactions are established across TAD boundaries in Drosophila embryos, but that developmental genes are strikingly enriched in intra- but not inter-TAD interactions. We pursued this observation using the twist locus, a master regulator of mesoderm development, and systematically relocated one of its enhancers to various genomic locations. While this developmental gene can establish inter-TAD interactions with its enhancer, the functionality of these interactions remains limited, highlighting the existence o

SUBMITTER: Balasubramanian D 

PROVIDER: S-EPMC10899756 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature