Chicken γδ T cells proliferate upon IL-2 and IL-12 treatment and show a restricted receptor repertoire in cell culture.

ABSTRACT: In chickens, γδ T cells represent a large fraction of peripheral T cells; however, their function remains largely unknown. Here, we describe the selective in vitro expansion of γδ T cells from total splenocytes by stimulation with the cytokines IL-2 and IL-12. Under these conditions, γδ T cells proliferated preferentially and reached frequencies of >95% within three weeks. Although IL-2 alone also triggered proliferation, an increased proliferation rate was observed in combination with IL-12. Most of the expanded cells were γδ TCR and CD8 double-positive. Splenocytes sorted into TCR1⁺CD8⁺, TCR1^highCD8^-, and TCR1^lowCD8^- subsets proliferated well upon dual stimulation with IL-2/IL-12, indicating that none of the three γ

SUBMITTER: Linti AE 

PROVIDER: S-EPMC10900522 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature