Physiological cell bioprinting density in human bone-derived cell-laden scaffolds enhances matrix mineralization rate and stiffness under dynamic loading.

ABSTRACT: Human organotypic bone models are an emerging technology that replicate bone physiology and mechanobiology for comprehensive in vitro experimentation over prolonged periods of time. Recently, we introduced a mineralized bone model based on 3D bioprinted cell-laden alginate-gelatin-graphene oxide hydrogels cultured under dynamic loading using commercially available human mesenchymal stem cells. In the present study, we created cell-laden scaffolds from primary human osteoblasts isolated from surgical waste material and investigated the effects of a previously reported optimal cell printing density (5 × 10⁶ cells/mL bioink) vs. a higher physiological cell density (10 × 10⁶ cells/mL bioink). We studied mineral formation, scaffold stiffness, and cell morphology ove

SUBMITTER: de Leeuw AM 

PROVIDER: S-EPMC10900528 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature