Equine enteroid-derived monolayers recapitulate key features of parasitic intestinal nematode infection.

ABSTRACT: Stem cell-derived organoid cultures have emerged as attractive experimental models for infection biology research regarding various types of gastro-intestinal pathogens and host species. However, the large size of infectious nematode larvae and the closed structure of 3-dimensional organoids often hinder studies of the natural route of infection. To enable easy administration to the apical surface of the epithelium, organoids from the equine small intestine, i.e. enteroids, were used in the present study to establish epithelial monolayer cultures. These monolayers were functionally tested by stimulation with IL-4 and IL-13, and/or exposure to infectious stage larvae of the equine nematodes Parascaris univalens, cyathostominae and/or Strongylus vulgaris. Effects were recorded using transcri

SUBMITTER: Hellman S 

PROVIDER: S-EPMC10900620 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature