Identifying Hmga2 preserving visual function by promoting a shift of Muller glia cell fate in mice with acute retinal injury.

ABSTRACT:

Background

Unlike in lower vertebrates, Müller glia (MG) in adult mammalian retinas lack the ability to reprogram into neurons after retinal injury or degeneration and exhibit reactive gliosis instead. Whether a transition in MG cell fate from gliosis to reprogramming would help preserve photoreceptors is still under exploration.

Methods

A mouse model of retinitis pigmentosa (RP) was established using MG cell lineage tracing mice by intraperitoneal injection of sodium iodate (SI). The critical time point for the fate determination of MG gliosis was determined through immunohistochemical staining methods. Then, bulk-RNA and single-cell RNA seq techniques were used to elucidate the changes in RNA transcription of the retina and MG at that time point, and new genes that may det

SUBMITTER: Yin Z 

PROVIDER: S-EPMC10900711 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature