Intermittent rapamycin feeding recapitulates some effects of continuous treatment while maintaining lifespan extension.

ABSTRACT:

Objective

Rapamycin, a powerful geroprotective drug, can have detrimental effects when administered chronically. We determined whether intermittent treatment of mice can reduce negative effects while maintaining benefits of chronic treatment.

Methods

From 6 months of age, male and female C3B6F1 hybrid mice were either continuously fed with 42 mg/kg rapamycin, or intermittently fed by alternating weekly feeding of 42 mg/kg rapamycin food with weekly control feeding. Survival of these mice compared to control animals was measured. Furthermore, longitudinal phenotyping including metabolic (body composition, GTT, ITT, indirect calorimetry) and fitness phenotypes (treadmil, rotarod, electrocardiography and open field) was performed. Organ specific pathology was assessed at 24 mon

SUBMITTER: Baghdadi M 

PROVIDER: S-EPMC10900781 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature